Imaging membrane potential in dendritic spines
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چکیده
منابع مشابه
Imaging membrane potential in dendritic spines.
Dendritic spines mediate most excitatory inputs in the brain. Although it is clear that spines compartmentalize calcium, it is still unknown what role, if any, they play in integrating synaptic inputs. To investigate the electrical function of spines directly, we used second harmonic generation (SHG) imaging of membrane potential in pyramidal neurons from hippocampal cultures and neocortical br...
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Neuronal activity causes substantial postsynaptic Na(+) transients. However, the physiological consequences of such Na(+) transients remain largely unknown. High-resolution Na(+) imaging is pivotal to study these questions, and, up to now, two-photon imaging with the fluorescent Na(+) indicator sodium-binding benzofuran isophthalate (SBFI) has been the method of choice. When introduced into ind...
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Excitatory input onto many neurons in the brain occurs onto specialized projections called dendritic spines. Despite their potential importance in neuronal function, direct experimental evidence on electrical signaling in dendritic spines is lacking as their small size makes them inaccessible to standard electrophysiological techniques. Here, we investigate electrical signaling in dendritic spi...
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متن کاملDendritic Spines
What are they? Dendritic spines are small protrusions that emerge from the dendrites of neurons (Figure 1). Most excitatory synapses in the brain are found on spine heads. Typically, a spine consists of a bulbous head, volume 0.001–1 μm3, at the end of a thin spine neck, diameter around 0.1 μm. A 100 μm length dendrite can contain several hundred spines. The human cerebral cortex is thought to ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2006
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0510092103